Author(s): Teixeira Marcos F. S. | Segnini Aline | Moraes Fernando C. | Marcolino-Júnior Luiz H. | Fatibello-Filho Orlando | Cavalheiro Éder T. G.
Journal: Journal of the Brazilian Chemical Society
ISSN 0103-5053
Volume: 14;
Issue: 2;
Start page: 316;
Date: 2003;
Original page
Keywords: modified carbon paste electrode | copper(II) hexacyanoferrate(III) | voltammetric determination | pharmaceutical preparations
ABSTRACT
A copper(II) hexacyanoferrate(III) (CuHCF) modified carbon paste electrode was used for the electroanalytical determination of pyridoxine (vitamin B6) in pharmaceutical preparations, using cyclic voltammetry. Diverse parameters were investigated for the optimization of the sensor response, such as composition of the electrode, electrolytic solution, effect of pH, scan rate of potential and interferences. The optimum conditions were found at an electrode composition of 20% CuHCF, 55% graphite and 25% mineral oil (m/m) in an acetate buffer (pH 5.5) containing 0.05 mol L-1 of NaCl. The range of determination of pyridoxine was from 1.2 x 10-6 to 6.9 x 10-4 mol L-1. The procedure was successfully applied to the determination of vitamin B6 in formulation preparations. The CuHCF modified carbon paste electrode gave results comparable to those obtained using spectrophotometry.
Journal: Journal of the Brazilian Chemical Society
ISSN 0103-5053
Volume: 14;
Issue: 2;
Start page: 316;
Date: 2003;
Original page
Keywords: modified carbon paste electrode | copper(II) hexacyanoferrate(III) | voltammetric determination | pharmaceutical preparations
ABSTRACT
A copper(II) hexacyanoferrate(III) (CuHCF) modified carbon paste electrode was used for the electroanalytical determination of pyridoxine (vitamin B6) in pharmaceutical preparations, using cyclic voltammetry. Diverse parameters were investigated for the optimization of the sensor response, such as composition of the electrode, electrolytic solution, effect of pH, scan rate of potential and interferences. The optimum conditions were found at an electrode composition of 20% CuHCF, 55% graphite and 25% mineral oil (m/m) in an acetate buffer (pH 5.5) containing 0.05 mol L-1 of NaCl. The range of determination of pyridoxine was from 1.2 x 10-6 to 6.9 x 10-4 mol L-1. The procedure was successfully applied to the determination of vitamin B6 in formulation preparations. The CuHCF modified carbon paste electrode gave results comparable to those obtained using spectrophotometry.
