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Development of Duplex PCR Assay for the Detection of Mycoplasma Gallisepticum and Mycoplasma Synoviae Prevalence in Pakistan

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Author(s): Abida Arshad | Khaleeq-Uz-Zaman | Ijaz Ali | Muhammad Arshad | Muhammad Saad Ahmed | Mukhtar Alam | Ahmad ur Rehman Saljoqi | Aqeel Javed | Naeemud Din Ahmad | Zahoor Ahmad Swati

Journal: International Journal of Animal and Veterinary Advances
ISSN 2041-2894

Volume: 5;
Issue: 3;
Start page: 114;
Date: 2013;
Original page

Keywords: Mycoplasma | Pakistan | pathogenic species | poultry

ABSTRACT
Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production of Pakistan. Timely and efficient diagnosis of the mycoplasma is needed in order to practice prevention and control strategies. A duplex Polymerase Chain Reaction (PCR) was optimized for simultaneous detection of two pathogenic species of avian mycoplasmas. Two sets of oligonucleotide primers specific for Mycoplasma gallisepticum and Mycoplasma synoviae, were used in the test. The developed assay exhibited high sensitivity and 100% specificity for the simultaneous detection of mycoplasma species prevalence in Pakistan.
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