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Development of efficient callus initiation of malta (Citrus sinensis) through tissue culture

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Author(s): Fazle Azim

Journal: International Journal of Agricultural Research, Innovation and Technology : IJARIT
ISSN 2224-0616

Volume: 1;
Issue: 1;
Start page: 64;
Date: 2011;
Original page

Keywords: Malta | Citrus sinensis | In vitro | Seed shoot formation | Callus induction

ABSTRACT
The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction.
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