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Development of an Electrogenerated Chemiluminescence Biosensor using Carboxylic acid-functionalized MWCNT and Au Nanoparticles

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Author(s): Ming-Hua Piao | Dae-Soo Yang | Kuk-Ro Yoon | Seung-Ho Lee | Seong-Ho Choi

Journal: Sensors
ISSN 1424-8220

Volume: 9;
Issue: 3;
Start page: 1662;
Date: 2009;
Original page

Keywords: Ru(bpy)32+ | Electrogenerated chemiluminescence | COOH-functionalized MWCNT | Au nanoparticle | ethanol | biosensor | real sample

ABSTRACT
A COOH-F-MWCNT-Nafion-Ru(bpy)32+-Au-ADH electrogenerated chemi-luminescence (ECL)electrode using COOH-functionalized MWCNT (COOH-F-MWCNT) and Au nanoparticles synthesized by the radiation method was fabricated for ethanol sensing. A higher sensing efficiency for ethanol for the ECL biosensor prepared by PAAc-g-MWCNT was measured compared to that of the ECL biosensor prepared by PMAc-g-MWCNT, and purified MWCNT. Experimental parameters affecting ethanol detection were also examined in terms of pH and the content of PAAc-g-MWCNT in Nafion. Little interference of other compounds was observed for the assay of ethanol. Results suggest this ECL biosensor could be applied for ethanol detection in real samples.
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