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Development of an Integrated Chip for Automatic Tracking and Positioning Manipulation for Single Cell Lysis

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Author(s): Chao-Wang Young | Jia-Ling Hsieh | Chyung Ay

Journal: Sensors
ISSN 1424-8220

Volume: 12;
Issue: 3;
Start page: 2400;
Date: 2012;
Original page

Keywords: electroosmotic flow | cell lysis | dielectrophoresis force | cell manipulation

ABSTRACT
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.
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