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Development of Moisture Stress Tolerant Brinjal cv. Utkal Anushree (Solanum melongena L.) Using Agrobacterium Mediated Gene Transformation

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Author(s): Deepti B. Sagare | I. C. Mohanty

Journal: Journal of Agricultural Science
ISSN 1916-9752

Volume: 4;
Issue: 8;
Date: 2012;
Original page

ABSTRACT
An efficient and reproducible in vitro regeneration protocol was developed with high frequency regeneration of shoot buds from shoot-tip in MS medium + BAP (2.0 mg/l) in Brinjal cv. Utkal Anushree. Rooting was obtained on MS medium+ NAA (0.01 mg/l). The Agrobacterium tumefaciens strain GV3107 containing a binary vector pCAMBIA2300 carrying rd29A::DREB1A gene has been used for transformation. The shoot-tip from in vitro grown seedling was pre-cultured for 72 hrs and co-cultivated for 24 hrs. Shoot buds were produced on the regeneration medium containing kanamycin (100 mg/l) and cefotaxime (250 mg/l). A transformation frequency of 6.40% was achieved after PCR analysis with gene specific primer. The gene expression for moisture stress tolerance was assessed through morpho-physiological and biochemical analyses.
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