Academic Journals Database
Disseminating quality controlled scientific knowledge

Development of a Stepping Force Analgesic Meter for a Rat Arthritic Model

ADD TO MY LIST
 
Author(s): Mun Fei Yam | Lip Yee Por | Kok Khiang Peh | Mariam Ahmad | Mohd. Zaini Asmawi | Lee Fung Ang | Delina Beh Mei Yin | Sim Ying Ong | Muthanna Fawzy Abdulkarim | Ghassan Zuhair Abdullah | Ibrahim Muhammad Salman | Omar Ziad Ameer | Elsnoussi Ali Hussin Mohamed | Mohd Akmal Hashim | Elham Farsi | Sook Yee Hor

Journal: Sensors
ISSN 1424-8220

Volume: 11;
Issue: 5;
Start page: 5058;
Date: 2011;
Original page

Keywords: arthritis | analgesimeter | nociception | stepping force | load cells

ABSTRACT
Behavioural assessment of experimental pain is an essential method for analysing and measuring pain levels. Rodent models, which are widely used in behavioural tests, are often subject to external forces and stressful manipulations that cause variability of the parameters measured during the experiment. Therefore, these parameters may be inappropriate as indicators of pain. In this article, a stepping-force analgesimeter was designed to investigate the variations in the stepping force of rats in response to pain induction. The proposed apparatus incorporates new features, namely an infrared charge-coupled device (CCD) camera and a data acquisition system. The camera was able to capture the locomotion of the rats and synchronise the stepping force concurrently so that each step could be identified. Inter-day and intra-day precision and accuracy of each channel (there were a total of eight channels in the analgesimeter and each channel was connected to one load cell and one amplifier) were studied using different standard load weights. The validation studies for each channel also showed convincing results whereby intra-day and inter-day precision were less than 1% and accuracy was 99.36–100.36%. Consequently, an in vivo test was carried out using 16 rats (eight females and eight males). The rats were allowed to randomly walk across the sensor tunnel (the area that contained eight channels) and the stepping force and locomotion were recorded. A non-expert, but from a related research domain, was asked to differentiate the peaks of the front and hind paw, respectively. The results showed that of the total movement generated by the rats, 50.27 ± 3.90% in the case of the male rats and 62.20 ± 6.12% in that of the female rats had more than two peaks, a finding which does not substantiate the assumptions made in previous studies. This study also showed that there was a need to use the video display frame to distinguish between the front and hind paws in the case of 48.80 ± 4.01% of the male rats and 66.76 ± 5.35% of the female rats. Evidently the assumption held by current researchers regarding stepping force measurement is not realistic in terms of application, and as this study has shown, the use of a video display frame is essential for the identification of the front and hind paws through the peak signals.
Why do you need a reservation system?      Affiliate Program