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DGAT1 p.K232A polymorphism in dairy and dual purpose Italian cattle breeds

Author(s): Emilio Scotti | Luca Fontanesi | Fausta Schiavini | Valeria La Mattina | Alessandro Bagnato | Vincenzo Russo

Journal: Italian Journal of Animal Science
ISSN 1594-4077

Volume: 9;
Issue: 1;
Start page: e16;
Date: 2010;
Original page

Keywords: Allele frequency | DGAT1 | Italian cattle breeds | QTN.

The aim of this study was to evaluate the allele frequency distribution at the DGAT1 p.K232A polymorphic site in seven Italian dairy and dual purpose cattle breeds. On the whole, 651 animals belonging Italian Holstein (116), Italian Brown (105), Italian Simmental (95), Valdostana Red Pied (95), Rendena (62), Reggiana (128) and Modenese (50) were genotyped by PCR-RFLP. Sequencing was carried out to confirm results of the genotyping protocol. The DGAT1 p.232K allele was identified in Italian Holstein (25.4%), Reggiana (17.2%), and with very low frequency in Italian Simmental, Valdostana Red Pied and Rendena (1%). In Italian Brown and Modenese, this allele was not detected. These results indicated that this polymorphic site can be considered for association studies only in Italian Holstein and Reggiana breeds. Deviation from Hardy-Weinberg equilibrium was observed in the Reggiana breed (P

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