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Differential Display Analysis of cDNA Involved in Microbial Mats Response after Heavy Fuel Oil Contamination

Author(s): Sylvain Bordenave | Marisol Goni-Urriza | Pierre Caumette | Robert Duran

Journal: Journal of Microbial & Biochemical Technology
ISSN 1948-5948

Volume: 01;
Issue: 01;
Start page: 001;
Date: 2009;
Original page

Keywords: Hydrocarbons | Microbial mats | Microcosms | mRNA | Differential display

Pristine microbial mats from Camargue salterns (France)maintained in microcosm were contaminated by Erika fueloil in order to identify gene sequences induced in responseto heavy fuel oil contamination. The differential displayapproach was adapted to detect differentially expressedmRNA in complex bacterial communities. Among the sixdifferentially expressed (DD) cDNA fragments isolated,one was identified and associated with an ABC-type effluxpump. A second DD-fragment was related to a conservedhypothetical protein found in many different bacterial species.Despite differentially expressed fragments could notbe clearly identified, this study reveals new perspectivesfor the improvement of our knowledge on the response ofmicrobial community after petroleum contamination.
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