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Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry

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Author(s): Yiyi Sun | Zhihe Zang | Xiaohong Xu | Zhonglin Zhang | Ling Zhong | Wang Zan | Yan Zhao | Lin Sun

Journal: International Journal of Molecular Sciences
ISSN 1422-0067

Volume: 11;
Issue: 4;
Start page: 1423;
Date: 2010;
Original page

Keywords: proteomics | hepatocellular carcinoma | serum biomarker

ABSTRACT
The aim of the current study is to identify the potential biomarkers involved in Hepatocellular carcinoma (HCC) carcinogenesis. A comparative proteomics approach was utilized to identify the differentially expressed proteins in the serum of 10 HCC patients and 10 controls. A total of 12 significantly altered proteins were identified by mass spectrometry. Of the 12 proteins identified, HSP90 was one of the most significantly altered proteins and its over-expression in the serum of 20 HCC patients was confirmed using ELISA analysis. The observations suggest that HSP90 might be a potential biomarker for early diagnosis, prognosis, and monitoring in the therapy of HCC. This work demonstrates that a comprehensive strategy of proteomic identification combined with further validation should be adopted in the field of cancer biomarker discovery.

Tango Jona
Tangokurs Rapperswil-Jona

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