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Disorders in local synthesis of growth factors in pylorus in children with infantile hypertrophic pyloric stenosis --- Zaburzenia lokalnej syntezy czynnikowwzrostu w odswierniku u dzieci z wrodzonymprzerostowym zwezeniem odswiernika

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Author(s): Janusz Jablonski | Renata Gawronska | Aleksandra Gawlowska | Ewa Andrzejewska

Journal: Przegląd Pediatryczny
ISSN 0137-723X

Volume: 37;
Issue: 1;
Start page: 27;
Date: 2007;
Original page

ABSTRACT
Introduction: Infantile hypertrophic pyloric stenosis (IHPS) in children is characterized by a progressive hypertrophy of pyloric muscle, the pathogenesis of which is not fully understood. There is increasing evidence suggesting that hypertrophy of the mu- scle and connective tissue remodeling is regulated by growth factors. Recent studies reported an increase in the local synthesis of some potent smooth muscle mitogens such as insulin-like growth factor-1 (IGF-1). However, there are no reports concerning local expression of platelet-derived endothelial cell growth factor (PDEGF), which plays an important role in the pathological angiogenesis. Aim of the study: The aim of this study was to analyse by immunohistochemistry (IHC) expression of IGF-1 and PDEGF in mu- scularis propria of the hypertrophic pyloric muscle in children with infantile hypertrophic pyloric stenosis (IHPS). Material and methods: Full-thickness muscle-biopsy specimens were obtained from 22 infants at the time of pyloromyotomy. Control group consisted of 7 children undergoing surgery because of other alimentary tract defects. Paraffin tissue sections were evaluated by routine histopathological methods and by immunohistochemistry using monoclonal mouse anti PDEGF or IGF-1 antibodies and polymer based peroxidase detections system. Morphometric analysis was performed using MultiScanBase V.808 analyzer. Cells showing positive reaction were counted in 5 random 200x high-power fields. Values were expressed as the mean +/-SD of the real expression area of the analyzed marker to the total analyzed area. Results: In children with IHPS the average area of PDEGF expression in the muscularis propria was 62 (+/-52.5) pkt/42 urn2, whereas in the control group it was 15 (+/-12.1) pkt/42 urn2. The average area of IGF-1 expression in the pyloric muscularis propria was 1037 (+/-491.9) pkt/42 urn2 in study group, whereas 259 (+/-221.44) pkt/42 urn2 in the control group. Statistically significant differences in the expression of PDEGF and IGF-1 in the pyloric muscularis propria were found between the children with IHPS and the control group (p

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