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Disruption of Yarrowia lipolytica biofilms by rhamnolipid biosurfactant

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Author(s): Dusane Devendra H | Dam Sushovan | Nancharaiah Yarlagadda V | Kumar Ameeta | Venugopalan Vayalam P | Zinjarde Smita S

Journal: Aquatic Biosystems
ISSN 2046-9063

Volume: 8;
Issue: 1;
Start page: 17;
Date: 2012;
Original page

Keywords: Biofilm | Biosurfactant | CTAB | Rhamnolipid | SDS | Yarrowia lipolytica

ABSTRACT
Abstract Background Yarrowia lipolytica is an ascomycetous dimorphic fungus that exhibits biofilm mode of growth. Earlier work has shown that biosurfactants such as rhamnolipids are efficient dispersants of bacterial biofilms. However, their effectiveness against fungal biofilms (particularly Y. lipolytica) has not been investigated. The aim of this study was to determine the effect of rhamnolipid on a biofilm forming strain of Y. lipolytica. Two chemical surfactants, cetyl-trimethyl ammonium bromide (CTAB) and sodium dodecyl sulphate (SDS) were used as controls for comparison. Results The methylene blue dye exclusion assay indicated an increase in fungal cell permeability after rhamnolipid treatment. Microtiter plate assay showed that the surfactant coating decreased Y. lipolytica biofilm formation by 50%. Rhamnolipid treatment disrupted pre-formed biofilms in a more effective manner than the other two surfactants. Confocal laser scanning microscopic studies showed that biofilm formation onto glass surfaces was decreased by 67% after sub-minimum inhibitory concentration (sub-MIC) treatment with rhamnolipids. The disruption of biofilms after rhamnolipid treatment was significant (P
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