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Distal element(s) is(are) required for position-independent expression of the goat α-lactalbumin gene in transgenic mice. Potential relationship with the location of the cyclin T1 locus

Author(s): Soulier Solange | Stinnakre Marie-Georges | Da Silva José | Lepourry Laurence | Mata Xavier | Besnard Nathalie | Vilotte Jean-Luc

Journal: Genetics Selection Evolution
ISSN 0999-193X

Volume: 32;
Issue: 6;
Start page: 621;
Date: 2000;
Original page

Keywords: insulator | α-lactalbumin | cyclin T1 | transgenesis | mice

Abstract We recently reported the site-independent and copy-number-related expression in mice of a goat α-lactalbumin gene with 150 kb and 10 kb of 5'- and 3'-flanking sequences, respectively. In the present study, we observed that the resection of the 5'-flanking region, leaving only 70 kb, resulted in a site-dependent expression of this milk protein-encoding transgene. This suggests that important cis-regulatory elements are located within the distal-deleted sequence. Within this region, we localised the promoter of the cyclin T1 gene, an ubiquitously expressed gene. So far, no other gene has been located between these two loci. Since these two genes are differentially expressed, our data suggest the potential location of an insulator within the deleted region that allows the two genes to be independently regulated.
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