Academic Journals Database
Disseminating quality controlled scientific knowledge

Distribution of alkaline and acid phosphatases in the duodenal wall of native blackgoats by using different fixatives

ADD TO MY LIST
 
Author(s): N. S. Ahmed

Journal: Iraqi Journal of Veterinary Sciences
ISSN 1607-3894

Volume: 24 (Arabic);
Issue: 2;
Start page: 169;
Date: 2010;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

ABSTRACT
Ten duodeni of adult goat were fixed in chilled acetone, 80% ethyl alcohol, alcohol-formalin solution, alcohol bouinssolution and buffered neutral formalin solution. The distribution of alkaline and acid phosphatases noticed in absorptive andgoblet cells that lining the duodenal mucosa of black goat, but different in their intensity and distribution according to differentfixatives. The distribution of alkaline phosphatase in absorptive columnar cells that lining intestinal glands was more intensethan other cells, whereas the concentration of acid phosphatase was more intense in goblet cells than other cells in the mucosaof goat duodenum specially in samples fixed in chilled acetone and ethyl alcohol 80%. The study revealed that the sampleswere fixed with chilled acetone gave highest reaction for alkaline and acid phosphatases than other fixative samples. Noreaction for alkaline and acid phosphatases included some absorptive cells lining villi, all cells lining the lower parts ofintestinal glands, paneth cells and submucosal glands in different fixatives, except submucosal glands revealed positivereaction for acid phosphatase in samples fixed in chilled acetone and 80% ethyl alcohol, paneth cells reveal positive reaction for the same enzyme in samples fixed in 80% ethyl alcohol in all examined areas of the duodenum wall of the native blackgoat.

Tango Jona
Tangokurs Rapperswil-Jona

     Save time & money - Smart Internet Solutions