Academic Journals Database
Disseminating quality controlled scientific knowledge

THE DNA-BINDING PROTEIN DPS FUNCTIONS AS A GLOBAL REGULATOR IN STARVED SALMONELLA ENTERICA SEROVAR ENTERITIDIS DURING STARVATION

ADD TO MY LIST
 
Author(s): LEONA NICOLE CALHOUN, JEONG NAM KIM, YUAN REN, JOON JIN SONG AND YOUNG MIN KWON

Journal: International Journal of Microbiology Research
ISSN 0975-5276

Volume: 3;
Issue: 3;
Start page: 136;
Date: 2011;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: Dps | gene regulation | Salmonella Enteritidis

ABSTRACT
The DNA binding protein of stationary phase cells (Dps)-first discovered in starved Escherichia coli-is capable ofproviding protection to cells during exposure to various environmental assaults. Its ability to do so is based on three intrinsicproperties of the protein: DNA binding, iron sequestration, and its ferroxidase activity. Proteomic studies have lead to theinference of a regulatory role for Dps as well; however, the ability of Dps to serve as a global regulator during nutritionaldeprivation has yet to be directly examined. In this study, we utilized microarray analysis and quantitative real-time PCR toestablish direct evidence for a regulatory role of Dps in starved Salmonella enterica serovar Enteritidis. The results of ourmicroarray screening revealed over 150 genes significantly up or down regulated in starved cells lacking functional Dpsprotein. Also, we identified a small subset of genes regulated by Dps that are important for the induction of hydrogenperoxide, iron, and acid resistance. The fact that it positively regulates genes important for stress resistance furthersolidifies Dps as a virulence regulator in S. enteritidis; for resistance to such cytotoxic conditions is likely to translate intoenhanced survivability and virulence within infected hosts.

Tango Jona
Tangokurs Rapperswil-Jona

     Affiliate Program