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DNA characterization of Mannheimia haemolytica A2, A7, A9 and Pasteurella trehalosi

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Author(s): M.Y. Sabri | M. Zamri-Saad | R. Son | Sharifah

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 4;
Issue: 2;
Start page: 210;
Date: 2005;
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ABSTRACT
Whole-cells DNA of Mannheimia haemolytica A2, A7, A9 and Pasteurella trehalosi, isolated from goats were subjected to plasmid screening and random amplified polymorphic DNA (RAPD) analysis. The plasmid DNA screening revealed the presence of plasmid only in P. trehalosi isolated from goat. The plasmid was 4825 bp. The RAPD analysis indicated similarities and differences among isolates M. haemolytica and P. trehalosi. However, OPA16 primer revealed the DNA fingerprinting pattern that was able to clearly distinguish the various isolated while OPA14 primer revealed less distinguishable pattern. OPA20 revealed a fairly distinguishable DNA pattern.
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