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Drug resistance of Staphylococcus aureus in sinusitis patients

Author(s): Sasikala Shanmugam1,2* , Ramganesh Selvarajan 1 , Sundararaj Thangiah 2

Journal: International Journal of Biosciences
ISSN 2220-6655

Volume: 1;
Issue: 3;
Start page: 63;
Date: 2011;
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Keywords: Sinusitis | nosocomial | Staphylococcus aureus | MRSA | mecA gene.

In this study on Sinusitis patients, we obtained 45 strains of Staphylococcus aureus. Antibiotic pattern of Staphyloccus aureus showed that resistance to Quinolones was 21% and 33% towards ciprofloxacin and oflaxacin respectively. Resistance to cephalosporins was 50% to cefuroxime, 41% and 50% to cefaperazone and cefotaxime respectively. Least resistance was noticed against aminoglycosides viz. Amikacin 47% and Gentamicin 21%. Resistance to Ampicillin and amoxicillin was 60% and 64% respectively. Oxacillin resistance was seen in 26% of the strains. Of the 45 isolates, 6 were found to be resistant for oxacillin . All these six isolates were subjected to Polymerase Chain Reaction (PCR) and they possessed the mecA gene. Correlation existed between the presence of mecA gene and oxacillin resistance in Staphylococcus aureusand these strains can be considered as MRSA and the patients can be advised for vancomycin therapy. Oxacillin resistance determination by phenotypic methods takes 24 hours to infer whereas PCR for mecAgene took only 6 hours. So the PCR techniques for the detection of mecA gene can be considered as gold standard (Rapid, Quick and accurate diagnosis) method for the detection of MRSA in spite of the cost involved.

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