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Dual Luciferase Assay: an Efficient Method to Study the Polyadenylation Process

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Author(s): Amer Jamil | Muhammad Yaqub | Munir A. Sheikh | Harold G. Martinson

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 3;
Issue: 9;
Start page: 1475;
Date: 2000;
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Keywords: ssay transcription | dual luciferase | polyadenylation | mRNA

ABSTRACT
Cleavage and polyadenylation is an obligatory step in mRNA bigenesis in eukaryotes. Poly(A) tails play an important role in mRNA turn over, transport and translatin. Currently available methods for the study of polyadenylation are very cumbersome and involve radioactivity. Dual luciferase assay was found to be an efficient and equally reliable method to CAT assay and RNase protection assay. Comparison of these methods is reported in this paper.
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