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Ecdysteroid 7,9(11)-dien-6-ones as potential photoaffinity labels for ecdysteroid binding proteins

Author(s): Pauline C. Bourne | Pensri Whiting | Tarlochan S. Dhadialla | Robert E. Hormann | Jean-Pierre Girault | Juraj Harmatha | René Lafont | Laurence Dinan

Journal: Journal of Insect Science
ISSN 1536-2442

Volume: 2;
Start page: 11;
Date: 2002;
Original page

Keywords: ecdysteroid receptor | affinity labelling | Ultraspiracle | Quantitative Structure-Activity Relationship | Comparative Molecular Field Analysis | 20-hydroxyecdysone | dacryhainansterone

Three ecdysteroid 7,9(11)-dien-7-ones (dacryhainansterone, 25-hydroxydacryhainansterone and kaladasterone) were prepared by dehydration of the corresponding 11alpha-hydroxy ecdysteroids (ajugasterone C, turkesterone and muristerone A, respectively). The biological activities of the dienones in the Drosophila melanogaster BII cell bioassay, which reflect the affinity for the ecdysteroid receptor complex, showed that the dienones retain high biological activity. Irradiation at 350 nm of the ecdysteroid dienones (100 nM) with bacterially-expressed dipteran and lepidopteran ecdysteroid receptor proteins (DmEcR/DmUSP or CfEcR/CfUSP), followed by loading with [3H]ponasterone A revealed that irradiation of dacryhainansterone or kaladasterone resulted in blocking of >70% of the specific binding sites. Thus, ecdysteroid dienones show considerable potential as photoaffinity analogues for ecdysteroid binding proteins.
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