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Efecto del 3-nitropropionato sobre el metabolismo del lactato y del acetato en neuronas y astrocitos crecidos in vitro en concentraciones perinatales

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Author(s): Jairo Alfonso Tovar- Franco | Ángel Alexandro Criollo-Rayo

Journal: Universitas Scientiarum
ISSN 0122-7483

Volume: 14;
Issue: 1;
Start page: 48;
Date: 2009;
Original page

Keywords: acetate | astrocyte | neuron | 3-nitropropionate | lactate

ABSTRACT
3-Nitropropionate effect on the lactate and acetate metabolism of neurons and astrocytes grown in vitro with perinatal concentrations.Anaplerotic reactions are an essential metabolic mechanism for the postnatal continuity of the brain development, contributing in processesthat require substrates synthesized from Krebs cycle intermediates; however, their role during the presuckling period in the neonate isunknown. Objective. To estimate the anaplerotic capacity of neurons and astrocytes grown in vitro under perinatal conditions. Materials andmethods. The effect of 3-nitropropionate (3-NPA)(2 mM) an inhibitor of the succinate dehydrogenase (SDH) on the oxidative andlipogenic metabolism of 14C-derived from acetate and lactate in perinatal concentrations. The results were compared with its respectivecontrols without inhibitor. Results. In spite of the presence of 3-NPA, respiratory activity with lactate was 40% in neurons and 73% inastrocytes, the lipogenesis was 53% in neurons and 52% in astrocytes. With acetate, the oxidation in neurons was 15% and 63% inastrocytes, lipogenesis was maintained in astrocytes but in neurons it increased up to 174% (p
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