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EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.

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Author(s): Bianca Perez Barcellos | Marco Antônio Rocha | Luciano Jamas | Felipe Zandonadi Brandão | Luis Garcia Nogueira | Tania Goes de Pinho

Journal: Ciência Animal Brasileira
ISSN 1518-2797

Volume: 12;
Issue: 1;
Start page: 126;
Date: 2011;
Original page

Keywords: Dogs | sperm | cooling.

ABSTRACT
The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC. No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.

Tango Jona
Tangokurs Rapperswil-Jona

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