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The effect of [Co(DH)2(An)2]2[TiF6]∙3H2O coordination compound on polypeptide spectrum of amylolytic preparation from Aspergillus niger 33-19 CNMN FD 02A strain

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Author(s): Alexandra CILOCI | Cezara BIVOL | Maria STRATAN | Veaceslav REVA | Steliana CLAPCO | Janeta TIURIN | Andrei RIJA | Eduard COROPCEANU

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XIX;
Issue: 2;
Start page: 128;
Date: 2012;
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Keywords: Aspergillus niger | α-amylase | enzyme purification | coordination compounds | SDS-PAGE

ABSTRACT
From mycelial fungus Aspergillus niger 33-19 CNMN FD 02A cultivated on nutrient medium supplemented with [Co(DH)2(An)2]2[TiF6]∙3H2O coordination compound amylolytic preparation with total α-amylase activity of 40042U/g and a specific activity of 131.3U/mg proteins was obtained. Through gel filtration and ion exchange chromatography enzyme preparation was purified 7.8 fold with a yield of 13%. SDS-PAGE electrophoresis of purified α-amylase revealed 2 polypeptide bands of 66 and 40.5kDa identified as α-amylase isomorphs similar to previously obtained results on Aspergillus niger grown without any coordination compounds. [Co(DH)2(An)2]2[TiF6]∙3H2O increase the activity of non-purified amylolytic preparation, most likely due to the bonds formed between metal complex and some α-amylase radicals.
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