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Effect of Age of Explant on Transgenic Cotton (Gossypium) Plant Due to Expression of Mannose-Binding Lectin Gene from Allium sativum

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Author(s): N.B. Afolabi-Balogun | H.M. Inuwa | S. Ibrahim | M.F. Ishiyaku | M.T. Bakare-Odunola | A.J. Nok | Lynelle van Emmenes

Journal: Asian Journal of Agricultural Sciences
ISSN 2041-3882

Volume: 3;
Issue: 5;
Start page: 393;
Date: 2011;
Original page

Keywords: Allium sativum | Blec 1 | cotton regeneration | explant regeneration | insecticidal lectin | transgenic plant

ABSTRACT
Cotton is the most important textile plant in the world and is one of the most important crops for the production of oilseed. Because of its worldwide economic importance, new cultivars are constantly being released in the world. Although great improvements have been achieved through traditional breeding methods, cotton breeders are facing many problems, i.e., narrow genetic base, inability to use alien genes and difficulty in breaking gene linkages. Genetic transformations analyses are main tools used by breeders to overcome these problems. The aim of the study reported in this paper is to determine the effect of age of explant on regeneration response of apical shoot for tissue culture and gene transfer systems of cotton. This enabled us evaluate it effects on cotton transformation. The age of explants was observed to have significant effect on shoot tip elongation. The elongation rates of the three varieties studied were not significantly different from each other (p = 0.1573) and was observed to be affected by the size of isolated tips. It was observed that if the starting size of the apex was less than 1 mm, the tips would not grow at all. Insecticidal lectin gene from Allium sativum was transferred into the transgenic cotton plants via Agrobacterium-mediated transformation using shoot apices as explants. Putative transgenic plants were confirmed by leaf GUS assay, kanamycin leaf test and molecular analysis of plantlet.
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