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EFFECT OF THE AQUEOUS EXTRACT OF BARK OF ALSTONIA SCHLOLARIS LINN. ON RESPIRATORY BURST IN POLYMORPHONUCLEAR NEUTROPHILS (PMNs)

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Author(s): Dalvi Alok Atmaram | Bhalerao Supriya Sudhakar | Munshi Renuka Premal

Journal: International Journal of Research in Ayurveda and Pharmacy
ISSN 2229-3566

Volume: 3;
Issue: 5;
Start page: 720;
Date: 2012;
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Keywords: Alstonia scholaris | Chemiluminescence assay | Nitrite estimation | Nitro blue Tetrazolium assay.

ABSTRACT
In the present study we explored the effect of aqueous extract of Alstonia scholaris bark as compared to known stimulant PMA (Phorbol 12-myristate 13-acetate), on the respiratory burst in Polymorphonuclear Neutrophils (PMNs). The formation of the various Reactive Oxygen Species (ROS) were measured by performing three in vitro assays viz. Nitro blue Tetrazolium (NBT) assay, Nitrite estimation and Chemiluminescence assay. The studied concentrations of Alstonia scholaris were (1, 5, 10 and 25 mg/ml) to evaluate efficacy. We observed an increase in the respiratory burst at all the studied concentrations in all the assays indicating its immunomodulatory effect. In case of superoxide radical generation and formation of ROS, we observed maximum increase with the lowest and highest concentrations (1 and 25 mg/ml respectively) as compared to two intermediate concentrations (5 and 10 mg/ml). While in case of nitrite formation, a concentration dependant increase was seen with maximum response at 25 mg/ml. These results were however not significant statistically as compared to either cell control or the standard drug, PMA. Our findings thus support the folklore use of the aqueous extract of Alstonia scholaris bark as immunomodulatory agent.
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