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EFFECT OF AUXINS AND CYTOKININS ON IN VITRO PROPAGATION OF IONIDIUM SUFFRUTICOSUM GING - A SEASONAL MULTIPOTENT MEDICINAL HERB

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Author(s): Sonappanavar Arunkumar B. | M. Jayaraj

Journal: International Journal of Research in Ayurveda and Pharmacy
ISSN 2229-3566

Volume: 2;
Issue: 1;
Start page: 198;
Date: 2011;
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Keywords: Callus | indirect regeneration | Ionidium suffruticosum | Kinetin | shoot multiplication.

ABSTRACT
The present work is to analyze the effect of auxins and cytokinins to develop a standard protocol for in vitro propagation of Ionidium suffruticosum Ging. Hormones like 2, 4-D, NAA, IBA, IAA, Kinetin, BAP and Zeatin were used in different combinations and concentrations for callogenesis and indirect plant regeneration. The rapid and maximum callus formation was achieved from leaf explant on MS medium supplemented with 2, 4-D (0.5mg/L). MS with 2,4-D (0.5mg/L) was a prompted media to maintain the leaf callus for a long duration and to possess highly regenerative potential referred to as “stock callus”. Different hormonal combinations often caused the transformation of the calli from soft crystalloid and translucent to compact, hard, yellowish and greenish forms. Maximum (95%) and highest number (40.67/explant) of multiple shoots were obtained from leaf callus in MS media containing Kinetin (4.0mg/L). Among the auxins and cytokinins used the maximum (62%) root formation in the shoots of leaf derived callus was achieved on the MS medium supplemented with NAA (2.0mg/L) and kinetin (0.2mg/L). However, the thick and longest roots (6.3cms) from shoot lets were obtained on MS medium with Kinetin (4.0mg/L). Seventy percent of the rooted plantlets were survived and successfully acclimatized in soil.

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