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The Effect of Crude Extract of Pandanus conoideus Lamb. var. Yellow Fruit on Apoptotic Expression of the Breast Cancer Cell Line (T47D)

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Author(s): OKID PARAMA ASTIRIN | MARTI HARINI | NOOR SOESANTI HANDAJANI

Journal: Biodiversitas
ISSN 1412-033X

Volume: 10;
Issue: 1;
Start page: 44;
Date: 2009;
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Keywords: T47D | Apoptotic | p53 | Pandanus conoideus Lamb. var. yellow fruit

ABSTRACT
A mechanism controlling a growing cancer cells is by a programmed cell death (apoptosis). The wildtype-p53 enable to stop cleaves that follow DNA repair or cell death (apoptosis). The mutation of wt-p53 caused loosing its ability to inhibit cancer cells proliferation. Healing methods like surgery, radiation, immunotherapy and chemotherapy still have some weaknesses, and clinical medicine to cancer is also still has any dissatisfactory. Much of chemotherapy was not given optimal result yet, because no specific action to cancer cells only, but also to the normal cells. These problems encourage important effort to find specific and sensitive anticancer. Empirical evidence indicates that the crude extract of Pandanus conoideus Lamb var. yellow fruit has potential effect as an anticancer. Method of Freshney was used in growing T47D cell line, counting cells was done by direct counting, and apoptotic evaluation was done by TUNEL enzymatic labeling assay. The results of the research demonstrated that the LC50 of yellow fruit extract are 0.25 µL/mL. The percentage of apoptotic of 0.125 µL/mL, 0.0625 µL/mL, and 0.03125 µL/mL are 34.38±2.26, 30.03±3.87 and 21.07±1.14 respectively.
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