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Effect of deuterium depleted water and pi water about in vitro germination of mature caryopses of some species

Author(s): Adriana PETRUŞ-VANCEA | Cristian-Felix BLIDAR | Iuliu LADÁNYI

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XVII;
Issue: 1;
Start page: 170;
Date: 2010;
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Keywords: cereals | micropropagation | deuterium depleted water | Pi water

In the present experiments we studied the capacity of three types of cereals – wheat (Triticum aestivum cv. Ariesan), rye (Secale cereale cv. Orizont) and their hybrid, triticale (Triticosecale Wittmack cv. Trilstar) - to be cultivated by specific methods based on vegetal biotechnologies, achieving “in vitro” cultures initiation, from mature caryopses, on culture mediums prepared with different types of water, respectively deuterium depleted water (with 25 ppm D) or Pi water, as the substitute of distilled water from the recipe elaborated by Murashige – Skoog (1962), by this observing the effects of these modifications in the composition of vitroculture medium, concerning the caryopsis germination. At the end of the experiment we found that, although in the first hours of germination, the deuterium depleted water, with 25 ppm (part per molecule) D – present in the culture medium – inhibits rootlets growing, at 5 days from the germination of three types of caryopses, on aseptic medium, substitution of distilled water, from the vitroculture medium, especially with deuterium depleted water, with 25 ppm D and in a smaller measure with Pi water, led to the significant stimulation, both of the germination process and plants growing resulted from germinated embryo, especially of its rootlets.
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