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The effect of diethyldithicarbarnate on antioxidant enzyme activities in the blood of rats

Author(s): Radojičić Ratko M. | Borković Slavica | Pavlović Slađan Z. | Nikolić Aleksandra L. | Blagojević Duško P. | Saičić Zorica S. | Spasić Mihajlo B.

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 52;
Issue: 5-6;
Start page: 329;
Date: 2002;
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Keywords: diethyldithiocarbamate | rats | blood | antioxidant enzymes.

Diethyldithiocarbamate (DDC) exhibits a variety of effects including neurotoxic, radio-protective and sensitizing activity. It is a potent copper chelating agent used for the treatment of oxygen toxicity, as an imunomodulator in cancer therapy, as well as in HIV infected patients. In this study we examined the effect of DDC, a superoxide dismutase (SOD) inhibitor, on the activities of copper-zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST). Three hours after diethyldithiocar-bamate treatment (1 g/kg b.m, i.p) a significant decrease of SOD and increase of GR and GST activities were found in the blood of rats. A negative correlation between SOD and GR and a positive correlation between GR and GST activities were also obtained. DDC induced a concentration dependent increase of GR activity and NADPH consumption in an enzymatic assay in vitro. The obtained results may be interpreted to indicate that a decrease in SOD activity in the blood of rats, after DDC administration, may be compensated for by changes in the activity of some other compounds suggesting that regulation of antioxidative defence is very complex.
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