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The effect of human recombinant leukemia inhibitory factor on sperm motility and survival

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Author(s): Saki Gh | Ghalambor Dezfully F | Sobhani A

Journal: Tehran University Medical Journal
ISSN 1683-1764

Volume: 65;
Issue: 9;
Start page: 1;
Date: 2007;
Original page

Keywords: Normal spermatozoa | motility | LIF | fallopian tube

ABSTRACT
Background: Leukemia inhibitory factor (LIF) is a group of secreted glycoproteins with molecular weights ranging from 38-67 kD, resulting from differential protein glycosylation. LIF is constitutively expressed at high levels in the human fallopian tube epithelium and has an important role in the motility and vitality of sperm. In the present study, the effect of human recombinant LIF on human sperm motility and survival in vitro was investigated.Methods: Normal spermatozoa of 30 fertile men were collected and after preparation were incubated in Ham's F10+FCS 10% medium, containing various concentrations (0, 3, 5, 10, and 50 ng/ml) of LIF at 37 ÂșC under 5% CO2 for 6, 24 and 48 hours. Sperm motion characteristics were measured using a Makler chamber. Sperm survival was determined using the hypoosmotic swelling test. Collected data were analyzed by one-way ANOVA and LSD test using SPSS version 11. The difference in values were considered significant when p
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