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Effect of Interaction of Ni2+ and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells

Author(s): LUO Zhen-Lan, WANG Hai-Bo, SU Jian-Sheng

Journal: Journal of Inorganic Materials
ISSN 1000-324X

Volume: 27;
Issue: 2;
Start page: 179;
Date: 2012;
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Keywords: dental casting alloy; green tea; Ni2+; EGCG: tongue carcinoma cells

Ni2+ is the major ion release from nickel―based dental casting alloy and carcinogens. The major polyphenolic constituent present in green tea, (―)epigallocatechin―3―gallate (EGCG), shows chemoprevention on human cancer. The aim of this study was to determine the interaction between EGCG and Ni2+ and the biological effects on tongue squamous cell carcinoma. The interaction of EGCG and Ni2+ was analyzed by LC/MS. The cell viability was assessed by the MTT reduction assay. The results of LC/MS indicated that [EGCG―2Ni2+] and [EGCG―Ni2+] formed when EGCG and Ni2+ were added simultaneously. Ni2+ and EGCG both inhibited the proliferation of tongue carcinoma cells in a time― and dose―dependent manner. The cytotoxicity against tongue carcinoma cells was increased when EGCG and Ni2+ cotreated, and the inhibition effects were extremely dependent on the concentrations of EGCG and Ni2+, addition order and the ratio of [Ni2+] to [EGCG].
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