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Effect of LIGHT Adjuvant on Kinetics of T-Cell Responses Induced by HSV-1 DNA Immunization

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Author(s): Masumeh Gorgian Mohammady | Taravat Bamdad | Masoud Parsania | Hoorieh Soleimanjahi | Somayeh Pouyanfard | Hamidreza Hashemi | Mohammad Asghari-Jafarabadi

Journal: Iranian Journal of Immunology
ISSN 1735-1383

Volume: 8;
Issue: 2;
Start page: 76;
Date: 2011;
Original page

Keywords: Activity | Cytotoxic T-Lymphocytes | HSV-1 | Infection | LIGHT

ABSTRACT
Background: Studies on efficacy of various vaccines that prevent or reduce the primary and recurrent HSV-1 infection have demonstrated the importance of cellular immunity for protection against the infection. We previously used DNA vaccination to induce cel-lular immunity against HSV-1 infection in mice. Objective: The aim of our study was to evaluate the effect of LIGHT, a member of TNF super family, on the kinetic of CTL response induced by HSV-1 glycoprotein B based DNA vaccine. Methods: Using a granzyme B ELISA for detection and analysis of CD8+ T cells, CTL activity was de-termined in the spleen of BALB/c mice at various time points after primary and booster dose of vaccination. The kinetics of CTL response to primary and secondary HSV-1 in-fection and DNA vaccination were compared to those induced by DNA vaccination in combination with LIGHT adjuvant in the present study. Results: In primary and secon-dary immunization, the CTL activity in the HSV injected group peaked 7 days and 12 hours post immunization, respectively. After 5 days, LIGHT could neither accelerate the CTL response compared to DNA vaccination alone nor could enhance the CTL activity in the primary and the first peak of memory response, the amount of granzyme B in-duced by the LIGHT containing vaccine was significantly higher than that induced by the vaccine without the adjuvant. Conclusion: Although LIGHT enhances the cellular response in the booster dose of vaccination, it does not accelerate the CTL response.

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