Academic Journals Database
Disseminating quality controlled scientific knowledge

Effect of Metal Ions, EDTA and Sulfhydryl Reagents on Soybean Amylase Activity

ADD TO MY LIST
 
Author(s): Om Prakash | Nivedita Jaiswal | Rajesh Kumar Pandey

Journal: Asian Journal of Biochemistry
ISSN 1815-9923

Volume: 6;
Issue: 3;
Start page: 282;
Date: 2011;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: partial purification | chelating reagents | detergent formulations | metal ions | Amylase

ABSTRACT
-1 protein) of soybean seeds were studied. Cobalt (II) and Manganese (II) exhibited marked activating effects on the activity, enhancing up to 200% of the initial activity at 2 mM concentration while Mercury (II) ions severely inhibited. Inhibition by mercury and activation by cobalt and manganese were concentration-dependent. However, other metal ions (K+, Ca2+, Mg2+, Al3+, Cu2+, Zn2+ and Fe3+) moderately increased the enzyme activity to a certain extent and then suppressed. Sodium, Cadmium and Nickel had no detectable influence on the activity. EDTA (12.5 mM) was found to be ineffective even for 1 h of incubation at 27C suggesting that no metal ion is present in the enzyme. No marked inhibition of amylase activity with the sulfhydryl reagents was found. The stability of the enzyme to metal ions as well as EDTA suggests its promising potential for use in detergent industries.]]>

Tango Rapperswil
Tango Rapperswil

     Save time & money - Smart Internet Solutions