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EFFECT OF METHOMYL ON PROTEASE ACTIVITY IN FREE AND IMMOBILIZED ESCHERICHIA COLI

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Author(s): KULKARNI AG AND KALIWAL BB

Journal: International Journal of Microbiology Research
ISSN 0975-5276

Volume: 3;
Issue: 2;
Start page: 74;
Date: 2011;
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Keywords: methomyl | protease | Escherichia coli | free cells | immobilized cells

ABSTRACT
Immobilized biocatalyst such as enzymes, microbial cells and cellular organelles, seem to be useful forcontinuous monitoring of the metabolites because of their high sensitivity and substrate specificity. The approach byconfining enzymes in a defined space is extremely useful for enzyme reutilization and particularly favorable for appliedbioprocess and it is apparent that Escherichia coli remains widely accepted as top choice among the bacterial cells. Freeand immobilized Escherichia coli were exposed to methomyl of concentrations ranging from 10-7 M to 10-3 M for a period of96 hrs and protease activity was evaluated at regular intervals of 24, 48, 72 and 96 hrs. The results indicated a closecorrelation between the toxic effects in free and immobilized cells. The protease activity increased significantly with anincrease in the dose and duration of exposure to methomyl in both free and immobilized cells, however, in comparison withits free counterpart the protease activity was less in immobilized Escherichia coli cells revealing that immobilized systemshows selective proteolysis and is less sensitive to the toxicant.

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