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The Effect of Nanozirconia Mixed with Glass-ionomer on Proliferation of Epithelial Cells and Adhesive Molecules

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Author(s): Hassan Semyari | Mandana Sattari | Mohammad Atai | Maedeh Pournasir

Journal: Journal of Periodontology & Implant Dentistry
ISSN 2008-7756

Volume: 3;
Issue: 2;
Start page: 63;
Date: 2011;
Original page

Keywords: Epithelium | glass-ionomer | ICAM-1 | zirconia

ABSTRACT
Background and aims. The aim of the present study was to evaluate the effect of nanoparticles of zirconiamixed with glass-ionomer on the proliferation of epithelial cells and adhesive molecules (ICAM-1).Materials and methods. Zirconia nanoparticles were mixed with glass-ionomer powder in weight percentages of 0%, 5%, 50%, 70%, and 100%. The powders were then mixed with glass-ionomer liquid in 2:1 weight ratios. The paste was then in-serted into a steel ring mold (5 mm in diameter and 0.5 mm in thickness) sandwiched between two glass slides. Glass-ionomer was then cured using a light-curing unit. Seven samples (discs) were prepared for each mixing percentage. Cell cultivation (epithelial) and MTT tests were performed to assess the cytotoxicity of specimens containing different nanozirconiacontents. Finally, human ICAM-1 platinum ELISA test was performed for quantitative diagnosis of human ICAM-1 epithelial cells.Results. Statistically significant differences (p
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