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The effect of Panton Valentin Leukocidin (PVL) on the airway smooth muscle viability

Author(s): Rodopi Stamatiou | Efthimia Petinaki | Apostolia Hatziefthimiou

Journal: Open Journal of Molecular and Integrative Physiology
ISSN 2162-2159

Volume: 03;
Issue: 01;
Start page: 42;
Date: 2013;
Original page

Keywords: Staphylococcus Aureus | PVL | Airway Smooth Muscle Cells

acute uncontrolled inflammation and tissue injury. n this study we investigated the effect of nimmunochromatographic test- 1:2000) for 1 - 3 days. The effect of PVL on the ASMC morphology or viability was estimated using microscope observations or indirect immunofluores- cence with anti-Smooth muscle -actin antibody and Dapi for DNA staining, and Trypan blue staining, respectively. ASMC incubated with PVL exhibit increased cell size, granular cytoplasm, and ruptured nuclei. Furthermore, PVL reduces cell number mainly in ASMC incubated in the presence of 10% FBS, therefore actively proliferating cells. These results show that apart from the known effect of PVL on immune cells and inflammation process, PVL has a direct toxic effect on airway smooth muscle cells.