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Effect of parenteral nutrition solutions on biofilm formation of coagulase-negative Staphylococci: An experimental study

Author(s): J. Sedef Göçmen | Ünase Büyükkoçak | Osman Çağlayan

Journal: Journal of Clinical and Experimental Investigations
ISSN 1309-8578

Volume: 3;
Issue: 4;
Start page: 505;
Date: 2012;
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Keywords: Catheter-related infections | biofilm | parenteral nutrition

Objectives: In our study we investigated the effects ofparenteral nutrition (PN) solutions on Coagulase negativestaphylococci (CoNS) biofilm production.Materials and methods: Thirty nine CoNS strains isolatedfrom hemocultures and a reference strain (ATCC 12228Staphylococcus epidermidis) were included. Bacterial dilutionswere made in Tryptic Soy Broth (TSB). The experimentalmediums were 1. Glucose, 2. Amino acid, 3. Lipid,4. Glucose+ Amino acid+ lipid, 5. Glucose+ Amino acid, 6.Glucose+ Lipid, 7. Amino acid+ Lipid, and 8. Control (TSB).Biofilm formation was evaluated by “quantitative microdilutionplaque test”. The values greater than cut off valueare considered as positive. Biofilm positivity was dividedinto 3 groups (mild, moderate and intensive) and all otherstrains under cutoff value were accepted as negative. Thenumbers of biofilm positive strains derived from 1-7. mediumswere compared with each other, and with the resultsof control.Results: The three-component PN solution and two componentPN solutions containing glucose+ lipid and aminoacid+ lipid were found to increase the biofilm productionactivity of CoNS when compared to the control group.Slime positivity in medium 1 and 2 was lower than controlsignificantly, in medium 4, 6, and 7 slime positivity washigher considerably. The indifferent results were obtainedwithin the mediums 1, 2, 3 and within the mediums 4, 5, 6,and 7.Conclusions: In our study, it was found that, glucose, aminoacid and lipid solutions which were building structuresof PN decreased the biofilm production when used solitary.However use of the compounds increased the biofilmproduction. Therefore, we can conclude that PN solutionsgiven as mixtures in routine practice increase the risk ofcatheter infection. J Clin Exp Invest 2012; 3(4): 505-509Key words: Catheter-related infections, biofilm, parenteralnutrition
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