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Effect of rootstock on the scion of Hevea brasiliensis through metabolic analysis of latex samples by 1H NMR

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Author(s): Eduardo Sanches Pereira do Nascimento | Clayton Rodrigues de Oliveira | Paulo de Souza Gonçalves | Reginaldo Brito da Costa | Rogério Manoel Biagi Moreno | Luiz Henrique Capparelli Mattoso | Antonio Gilberto Ferreira

Journal: Crop Breeding and Applied Biotechnology
ISSN 1518-7853

Volume: 11;
Issue: Special;
Start page: 82;
Date: 2011;
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Keywords: metabolic profile | plant metabolomics | chemometric analysis | nuclearmagnetic resonance.

ABSTRACT
In this study, the effect of rootstock on grafting through metabolomic analysis of latex (Hevea brasiliensis) samples was verified by 1H nuclear magnetic resonance (NMR) and multivariate data analysis. Sixteen metabolites present in the latex cytosol were characterized by NMR. PCA analysis showed that the latex samples of the RR and GR groups can be differentiated. The GR group samples present a metabolic profile similar to the RR group samples, while the RG group is in an intermediate position between RR and GG groups. Sucrose and formate contributed greatly to the separation obtained by PCA, presenting a good correlation between the results. 1H NMR was an efficient technique to differentiate latex samples from different types of rootstocks and grafting and in the future could be used to predict rubber production by latex analysis.
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