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Effect of Semen Collection in Tris Extender Supplemented with Hypotaurine and Cysteine on Characteristics of Cooled and Post Thaw Boer Goat Spermatozoa

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Author(s): Akeel Ahmed Memon | H. Wahid | Y. Rosnina | Y.M. Goh | M. Ebrahimi | F.M. Nadia | A.H. Shah

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 11;
Issue: 9;
Start page: 1486;
Date: 2012;
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Keywords: Goat | sperm | hypotaurine | cysteine | cryopreservation | Malaysia

ABSTRACT
The objective of this study was to investigate whether pre freezing and post thawing (motility, morphology, viability, acrosome integrity and membrane integrity). Boer goat spermatozoa are affected by collecting in an Tris citric acid fructose extender supplemented with hypotaurine or cysteine. Semen was collected from four bucks into tubes containing 0 mL extender (Contro I), 2 mL extender without supplementation of antioxidants (Control II), 2 mL extender supplemented with hypotaurine 10 mM and 2 mL extender with cysteine 5 mm. No significant (p>0.05) improvement was observed by adding extender into collection tubes either supplemented or not with antioxidants before freezing and after freeze thawing. While a slight improvement was observed in motility, membrane integrity and acrosome integrity when 2 mL Tris citric acid fructose extender supplemented with either hypotaurine or cysteine. In conclusion, addition of extender in collection tubes either supplemented or not with antioxidants is not beneficial for Boer goat semen cryopreservation.
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