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The effect of some metal complexes of oxime ligands on proteolytic activity of Fusarium gibbosum CNMN FD 12 strain

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Author(s): Steliana CLAPCO | Cezara BIVOL | Alexandra CILOCI | Maria STRATAN | Eduard COROPCEANU | Janeta TIURIN | Andrei RIJA | Svetlana LABLIUC | Ion BULHAC

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XX;
Issue: 1;
Start page: 53;
Date: 2013;
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Keywords: Fusarium gibbosum (Gibberella intricans) | neutral and acid protease | coordination compounds | oxime ligands

ABSTRACT
The supplementation of Co(III) and Cu(II) coordination compounds to the nutrient medium of Fusarium gibbosum CNMN FD 12 essentially increases fungal proteolytic activity. The maximal stimulatory effect was obtained on the 5th day of cultivation. Depending on type and concentration, the compounds stimulated the activity of neutral proteases with 17.5-96.0% and the activity of acid proteases with 12.1-71.7%. On the 6th day of cultivation fungal proteolytic activity decreased significantly, in most cases below the control sample. The direct supplementation of coordination compounds to cultural liquid containing enzyme indicated a neutral or inhibitory effect of just 21.2% compared to the control. This demonstrated the indirect stimulatory effect of coordination compounds on proteolytic activity, most likely due to the inclusion of them in cell metabolism and not direct - in enzyme structure. The chelating reaction with EDTA showed 81.8-78.8% remaining activity of neutral proteases at concentration of 1-10mM EDTA. This result allows us to suppose that most F. gibbosum exoproteases are not metalloproteases. They can belong to family of extracellular serine proteases and aspartic proteases.
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