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Effect of trapidil on the sciatic nerve with crush injury: a light microscopic study

Author(s): Kurtoglu Z | Ozturk AH | Bagdatoglu C | Turac A | Camdeviren H | Uzmansel D | Aktekin M

Journal: Neuroanatomy
ISSN 1303-1783

Volume: 3;
Issue: 1;
Start page: 54;
Date: 2004;
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Keywords: anatomy | neuroanatomy | trapidil | crush | injury | myelin | sheath | sciatic | nerve

Trapidil’s therapeutic effect is shown in nervous tissue in ischemia and reperfusion injury, butany study on trapidil’s effects on regeneration in the peripheric nervous system after crushinjury is not encountered in the literature.In this study, 40 female albino wistar rats were used. The sciatic nerves were crushed for20 seconds by a jeweler’s forceps. A single dose of 8 mg/kg of trapidil was administered tothe treatment group intraperitoneally. After the crush injury, the crush site was excised on2nd, 7th, 15th, 30th, and 45th days, fixed in formalin and then prepared for routine histologicalevaluation. Each section was stained with toluidin blue. Myelin thicknesses were measuredby an ocular micrometer.Measurements were evaluated by factorial analysis of variance. Separation of myelin lamellaeand vacuole formation, which are signs of axonal degeneration were seen on the 7th and 15thdays of both groups, but were more prominent in the crush group. Regenerating myelinatedfibers were increased on the 30th and 45th days in increasing density. Interaction for myelinthickness was statistically significant with Student-Neyman-Keuls Post Hoc test (p = 0.038).In the trapidil group, myelin thickness was less on the 15th and 45th days according to thecontrol group (p < 0.05).These findings were interpreted as trapidil was effective in preventing edema and myelindamage by preventing vasospasm, inactivating macrophages, inhibiting the inflammatoryresponse and stabilizing the cell membrane. On the other hand, it is thought that trapidil hada retarding effect on myelin regeneration in the recovery period.

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