Academic Journals Database
Disseminating quality controlled scientific knowledge

The effects of aspirin on apoptosis of neutrophil granulocytes

ADD TO MY LIST
 
Author(s): Vasilev S. | Majstorović Ivana | Gašić Sonja | Vučević Dragana | Vasilijić S. | Ćupić Vitomir | Čolić M.

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 56;
Issue: 5-6;
Start page: 413;
Date: 2006;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: aspirin | neutrophil granulocytes | apoptosis

ABSTRACT
Neutrophils are a part of the immune system, and they are involved in host defence against microorganisms. Neutrophil granulocytes have the shortest lifespan among leukocytes, which can be modulated by cytokines and pharmacological agents. The effect of aspirin on apoptosis of inflammatory granulocytes has not been studied in detail yet, and therefore was the chosen subject of this study. Inflammatory granulocytes have been isolated from polyvinyl sponges implanted under the skin of Albino Oxford (AO) rats. Inflammatory cells that were isolated 20 hours later were more than 95% neutrophil granulocytes. The cells were cultivated 24 h with different concentrations of aspirin ranging from 1 µM to 10 mM. After the cultivation period, apoptosis of neutrophils was assessed by morphological criteria, as well as by flow cytometry (after staining the cells with propidium iodide). We found that at concentrations from 0,1 mM to 2,5 mM aspirin inhibited apoptosis of granulocytes, but at 10 mM aspirin induced apoptosis of these cells.
Save time & money - Smart Internet Solutions      Why do you need a reservation system?