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The effects of culture medium composition on in vitro rooting of two intergeneric hybrids Fragaria x Potentilla

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Author(s): Anca Nicoleta SUTAN | Aurel POPESCU | Valentina ISAC

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XVI;
Issue: 2;
Start page: 150;
Date: 2009;
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Keywords: in vitro culture | root induction | auxins | cytokinins | intergeneric hybrids Fragaria x Potentilla

ABSTRACT
As an important stage in micropropagating ornamental strawberry, in vitro rooting of microshoots on media containing different concentrations of auxins was investigated in two intergeneric hybrids Fragaria x Potentilla, respectively “Pink Panda” and “Serenata”. IBA at either 0.25 or 0.5 mg/l, and IAA at 0.5 mg/l concentration, were added to solidified Murashige and Skoog (1962) basal medium containing half strenght macroelements and half Lee-Fossard microelements. In all treatments, 0.1 mg/l of GA3 was also added to the basal medium. IBA was found to be the most effective auxin in promoting rhizogenesis, with the concentration 0.25 mg/l giving the highest rooting rates for both varieties, respectively 100% for “Pink Panda”, and 80% for “Serenata”.
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