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Effects of different concentrations of juglone on the proliferation and apoptosis of HeLa cells

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Author(s): Wei ZHANG | Xing-yu ZHAO | Yan LI | Jun-jie XU | Yan-xia JIANG | Shi-jie LV

Journal: Medical Journal of Chinese People's Liberation Army
ISSN 0577-7402

Volume: 38;
Issue: 2;
Start page: 99;
Date: 2013;
Original page

Keywords: juglone | hela cells | apoptosis | cell cycle

ABSTRACT
Objective  To study the effect of different concentrations of juglone on the proliferation and apoptosis of human cervical cancer cells (HeLa), and explore the anti-tumor effect of juglone in vitro. Methods  HeLa cells were cultured in vitrofor 24 h and then divided into control group and juglone-treated group. The cells in juglone-treated group were cultured again with 10, 20, 50, 100 and 200μmol/L juglone, respectively, for 24h. The morphological changes in HeLa cells were observed with inverted microscope. The proliferation of HeLa cells was assessed detected by methyl thiazolyl tetrazolium (MTT) assay. The cell cycle and apoptosis were observed by flow cytometry (FCM). The expression of caspase-3 in HeLa cells was determined by caspase-3 colorimetric assay kit. Results  Under morphological observation, it was found that different concentrations of juglone led to change in cell shape. MTT assay showed that juglone significantly inhibited the growth of HeLa cells in a dose-dependent manner (P
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