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Effects of Feiyanning on the Expression of Epithelial-mesenchymal Factors 
in Highly Metastatic Lung Cancer Cells 95-D

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Author(s): Xiaozhen ZHAO | Zhonghua WU | Zhenye XU | Zhongqi WANG | Ji WU | Wan SU

Journal: Chinese Journal of Lung Cancer
ISSN 1009-3419

Volume: 14;
Issue: 6;
Start page: 467;
Date: 2011;
Original page

Keywords: Lung neoplasms | Epithelial-mesenchymal transition | Alpha-catenin | beta-catenin | E-Cadherin | N-cadherin | Fibronectin | Vimentin | Tumor metastasis

ABSTRACT
Background and objective Our previous in vivo experiment (paper has been published) has confirmed Feiyanning’s regulation effect on epithelial cell markers Alpha-catenin, beta-catenin, E-Cadherin and mesenchymal cell markers N-cadherin, Fibronectin, vimentin. Based on previous study, the objective of this study is to further investigate the expression of epithelial-mesenchymal cell marker gene and protein intervened by Feiyanning in highly metastatic lung cancer cells 95-D in vitro. Methods Human highly metastatic lung cancer 95-D cells were treated with different concentrations of Feiyanning in vitro, and then Real-time PCR and Western blot methods were used to detect mRNA and protein expression of epithelial-mesenchymal cell marker factor Alpha-catenin, beta-catenin, E-Cadherin, N-cadherin, Fibronectin and vimentin. Results Real-time PCR results showed that compared with the control group, Feiyanning at 20% concentration remarkably up-regulated the expression of Alpha-catenin (P
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