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Effects of folate supplementation on cleft palate induced by lamotrigine or cycloposphamide: an experimental study in mice

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Author(s): Prakash | Prabhu LV | Singh G

Journal: Neuroanatomy
ISSN 1303-1783

Volume: 6;
Issue: 1;
Start page: 12;
Date: 2007;
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Keywords: cleft palate | cyclophosphamide | folic acid | lamotrigine | mice

ABSTRACT
This study aims to elucidate the preventive role of folate supplementation on induction of cleft palate in mice bydrugs of two separate categories i.e. lamotrigine (newer antiepileptic and antipsychotic) and cyclophosphamide(anticancer and immunosuppressive). 10 pregnant swiss white mice (C) received normal saline intraperitoneally on day 10 of gestation. Two groupsof 10 pregnant mice (T1) and (T2) each received lamotrigine or cycloposphamide respectively 10 mg/kg bodyweight (bw) intraperitoneally on day 10 of gestation. Folate was supplemented 0.8 μg/kg bw intraperitoneallyalong with lamotrigine or cyclophosphamide to two more groups of 10 pregnant mice (T3) and (T4) eachrespectively on the same day 10, of gestation. Fetuses were collected by Caesarian Section on day 18 ofgestation. Fetuses collected from all the groups were examined macroscopically with stereomicroscope forpalatal malformations. Coronal sections of fetal head were taken for histological study of palatine defects.Cleft palates were detected in 42 out of 70 (60.00%) fetuses of lamotrigine treated group (T1) and 49 out of 61(80.33%) fetuses of cyclophosphamide treated group (T2). Folate supplementation resulted in different response;15 out of 72 (20.83%) fetuses in T3 group and 51 out of 64 (79.69%) fetuses in T4 group had cleft palate. Thedifference was highly significant (p0.05) when it was administered with cyclophosphamide (T4) as compared to only lamotrigine(T1) or cyclophosphamide (T2) treated groups respectively. The preventive efficacies of folate supplementation for cleft palate vary considerably and in the same subjectunder identical conditions, depend primarily on the mechanism of action of the inducing agent.
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