Academic Journals Database
Disseminating quality controlled scientific knowledge

Effects of Glucose on Differentiation and Fat Metabolism of Chicken Preadipocytes

ADD TO MY LIST
 
Author(s): Qi Renli | Sun Chao | Yan Jun | Yang Haili | Zhao Taotao | Zhao Xia

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 11;
Issue: 8;
Start page: 1223;
Date: 2012;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: Glucose | chicken | preadipocyte | fat metabolism | cell differentiation | fatty acid transporter

ABSTRACT
The present study was carried out to illustrate the effect of glucose on chicken (Gallus gallus) preadipocyte differentiation and fat metabolism. Adipocyte differentiation was initiated by maintaining confluent cells in a glucose-free medium supplemented with different concentrations of glucose. Upon exposure to high concentrations of glucose (25 mmol L-1), Peroxisome Proliferator-Activated Receptor γ (PPARγ) and CCAAT/Enhancer-Binding Protein α (C/EBPα) as adipocyte differentiation markers were significantly increased compared with control cells. The morphology of the glucose-treated cells changed from fibroblast-like to polygonal and cells treated with moderate concentrations of glucose (15 mmol L-1) accumulated the most number of cytoplasmic lipid droplets as estimated by Oil red O staining. Compared with control cells varying concentrations of glucose affected the mRNA expression and protein levels for Fatty Acid Synthesis (FAS) and Adipose Triglyceride Lipase (ATGL), the master regulators of fat metabolism. Moreover, the mRNA expression levels and protein contents of fatty acid transporters in glucose-treated cells were higher than in untreated cells. These results indicated that glucose is essential material for chick adipocytes differentiation. Moreover, overhigh concentrations of glucose maybe stimulate lipolysis in the cells.
Why do you need a reservation system?      Affiliate Program