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Effects of microcystin-LR in isolated perfused rat kidney

Author(s): Nobre A.C.L. | Jorge M.C.M. | Menezes D.B. | Fonteles M.C. | Monteiro H.S.A.

Journal: Brazilian Journal of Medical and Biological Research
ISSN 0100-879X

Volume: 32;
Issue: 8;
Start page: 985;
Date: 1999;
Original page

Keywords: microcystin-LR | kidney | perfused kidney

Microcystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR (1 µg/ml) was perfused over a period of 120 min, during which samples of urine and perfusate were collected at 10-min intervals to determine the levels of inulin, sodium, potassium and osmolality. We observed a significant increase in urinary flow with a peak effect at 90 min (control (C) = 0.20 ± 0.01 and treated (T) = 0.32 ± 0.01 ml g-1 min-1, P
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