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Effects of microwave radiation on morphology and function of hematopoietic system of rats

Author(s): Wei SONG | Rui-yun PENG | Ya-bing GAO | Shui-ming WANG | Jing ZHANG | Li ZHAO | Xiang LI | Yang LI | Ji DONG

Journal: Medical Journal of Chinese People's Liberation Army
ISSN 0577-7402

Volume: 36;
Issue: 3;
Start page: 297;
Date: 2011;
Original page

Keywords: microwaves; rats; bone marrow

Objective To explore the effects of microwave radiation on the morphology and function of hematopoietic system in rats.Methods One hundred and forty-four male Wistar rats randomly divided into 4 groups(36 each) were exposed to microwave radiation at the average power density of 0,2.5,5 and 10 mW/cm2 respectively(0 mW/cm2 as sham group),with one exposure of 6 minutes,5 times per week,up to a total of 30 days.Six rats of each group were respectively sacrificed at 6 hours and 7,14,30,60 and 180 days post radiation to obtain blood samples in the inferior vena cava for counting leukocytes,erythrocytes and platelets,and detecting the concentration of hemoglobin.Additionally,bone marrow in sternum was obtained from sacrificed rats of each group for evaluation of histological and ultrastructural changes by light and electron microscopy.Results Compared with the sham group,a reduction,in different degree,was seen in numbers of white cells,neutrophils and erythrocytes 6 hours after radiation in 5mW/cm2 group,while a raise was seen in numbers of erythrocytes and platelets 60 days after radiation.At 180 days post radiation,a significant decrease was seen in total number of leukocytes and numbers of lymphocytes and platelets in 5mW/cm2 and 10mW/cm2 groups when compared with the sham group.Under light microscope,no dramatic changes was seen in the hematopoiesis cells of bone marrow at day 14 post radiation,obvious hyperemia and edema of interstitial tissue were seen at day 30 post radiation,while a reduction in hematopoiesis cells and an increase in adipocytes,both in a dose dependent manner,were seen at day 180.At day 7 post radiation,apoptosis and necrosis was seen in trilineage hematopoietic cells of bone marrow of 5mW/cm2 group under electron microscope.Conclusion A Long-term exposure to the microwave,ranging from 5 to 10mW/cm2,will result in morphological and functional changes,in a dose-dependant manner,in the hematopoietic system of rats.
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