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Effects of Polygonum Aviculare Herbal Extract on Proliferation and Apoptotic Gene Expression of MCF-7

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Author(s): M. Habibi Roudkenar | A. Mohammadi Roushandeh | A. Delazar | R. Halabian | J .Soleimani Rad | A. Mehdipour | M. Bagheri | A .Jahanian-Najafabadi

Journal: DARU : Journal of Pharmaceutical Sciences
ISSN 1560-8115

Volume: 19;
Issue: 5;
Start page: 326;
Date: 2011;
Original page

Keywords: Herbal Medicine | Breast Cancer | Knotweed | Cell Death

ABSTRACT
Background and the purpose of the study: One of the most common malignancies in women is breast cancer. Although several treatments for breast cancer are available, application of herbal medicine as a supplementary treatment is a new option to help curing the disease. In this study anticancer effects of Polygonum avicular herbal extract was investigated.Methods : Polygonum avicular extract was obtained by methanol. MCF-7 cell line was treated with different concentrations of Polygonum avicular (50, 100, 150, 200, 250, 300,350 400 ng/μl) for different time lengths ( 6, 12, 24, and 48 hrs). MTT assay and Flow Cytometry were used to evaluate cell proliferation and apoptosis, respectively. RT-PCR was also carried out to evaluate the expression of apoptotic genes. Results and Discussion: Results showed that Polygonum avicular induced cytotoxicity in MCF- 7 cell line at concentrations higher than 300 ng/μl and this was confirmed by the highest rate of cell death as measured by Trypan Blue and MTT assays. RT-PCR results showed up-regulation of P53 and down-regulation of Bcl-2 proteins which implied the ability of Polygonum avicular to induce apoptosis in MCF-7 cells and confirmed its anticancer property. Further studies are required to evaluate effects of the extract on other apoptotic genes.
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