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EFFECTS OF PROPOLIS EXTRACTS IN CHICKENS DIET AGAINST SALMONELLA TYPHIMURIUM DETECTED BY REAL-TIME PCR

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Author(s): Jaroslav Pochop | Miroslava Kačániová | Lukáš Hleba

Journal: Journal of Microbiology, Biotechnology and Food Sciences
ISSN 1338-5178

Volume: 1;
Issue: 2;
Start page: 113;
Date: 2011;
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Keywords: Real-time PCR | Salmonella spp. | detection kit | chicken | propolis

ABSTRACT
The aim of this study was to follow the effect of propolis extractsin chickens feed against colonization of GIT (gastrointestinal tract) with Salmonella spp. by Step One real-time PCR. Propolis has been used in folk medicine since ancient times due to its many biological properties, such as antimicrobial, anti-inflammatory, antioxidant, immunomodulatory activities, among others. Propolis extracts was applied to chickens feeds in four groups with different concentration of propolis. We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Salmonella spp. Detection Kit for pursuance the real-time PCR(Applied Biosystems). In the investigated samples without incubation we could detect strain of Salmonella spp. in twenty of twenty five samples (swabs), as well as internal positive control (IPC), which was positive in all samples. Our results indicate positive effect of propolis against colonization of GIT (gastrointestinal tract) with Salmonellaspp. in all experimental groups. This Step One real-time PCR assay is extremely useful for any laboratory equipped by real-time PCR. Thus, these results proved real-time PCR to be useful as a rapid diagnostic test for the direct detection of pathogens in food, without the need of enrichment steps.
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